Specifications:

• Accelerating Voltage: 300 kV, enabling high electron penetration for specimens up to ~300–600 nm thick.
• Electron Source: Field Emission Gun (X-FEG) with energy spread of ~0.7 eV
• Optics: C-TWIN three-condenser-lens system (constant-power) with parallel illumination; minimum beam size ~0.55 µm.
• Throughput: Aberration-Free Image Shift (AFIS), supporting ~700 images/hour.
• Cryo Handling: 12-grid autoloader for fully automated sample loading.
• Detectors: Gatan K3 direct electron detector paired with a Gatan BioContinuum energy filter.
• Software & Automation: EPU for automated single-particle acquisition, Tomography packages for cryo-ET workflows and Remote operation and monitoring capability

Applications: High-resolution single particle cryoEM and Cryoelectron tomography (cryoET)

Specifications:

• Accelerating Voltage: 20–120 kV
• Electron Source: Lanthanum hexaboride (LaB₆)
• Camera: Eagle 4 megapixel CCD 2048 × 2048 pixel resolution, Pixel size: 30 × 30 µm², 16-bit dynamic range and high detective quantum efficiency (DQE) for low-dose imaging
• Sample Holder: Single-tilt holder

Applications: Preparatory analysis of sample quality, particle distribution, and structural integrity for high-resolution cryo-EM workflows

Specifications:

• User Interface: Intuitive touchscreen interface and fully programmable protocols for reproducibility across sessions
• Environmental Control: Temperature range: 4 °C to 60 °C, Humidity control: up to 100% relative humidity (RH).
• Blotting System: Dual blotting pads with adjustable blot force, time, and offset. User-defined drain and wait times for optimal ice thickness control

Applications: Routine sample vitrification for single-particle cryoEM and cryoET.  

Specifications:

• Temperature Range: −15 °C to −185 °C in cryomode (FC7 cryo chamber).
• Section Thickness: Nominal feed range: 1 nm up to 15 µm.
• Cutting Speed: 0.05 – 100 mm/s.
• Eucentric Movement: Stereo-microscope carrier has eucentric movement, enabling precise approach of both glass and diamond knives in cryo mode.
• Cryo Modes: Three modes: standard, high gas flow (to reduce ice build-up), and wet sectioning (for cryoprotectants).
• Micromanipulator: Optional micromanipulator for precise collection of cryo-sections, e.g., on TEM grids.
• User Interface: Touch-screen control panel for section feed, cutting speed, temperature, auto-trim, and LN₂ level monitoring. 

Applications: Ultra-thin cryo-sectioning of biological samples for TEM, SEM, and CLEM.

Specifications:

• User Interface: Intuitive touchscreen interface that displays system readiness, LN₂ levels, and freezing parameters at a glance.
• Fast System Start-Up: 20-minute cooldown before the system is sample ready.
• Rapid Sample Vitrification Cycle: 1-minute recovery between each sample vitrification cycle.

• Sample Storage: Freezing process is fully automated including the deposition of up to 9 samples into the sample dewer. The sample dewer is also automatically refilled with LN2 as necessary.

   

Applications:  Sample preparation instrument that uses high pressure to vitrify thick tissue samples (up to ~300 µm) or alternatively produce Waffle grids for cross sections of thin samples for cryoET analysis. 

Specifications:

• GentleBlot Technology: Unique blotting system that provides gentle 1- or 2- side blotting for vitrifying cryo-EM samples.
• Ethane Temperature Controller: Built in heating system that keeps ethane just above its melting point to prevent ethane freezing.
• Electronic Blotting Timer: Blotting timer accurate down to 0.1 sec for precise blotting times. 

• Fully Enclosed Working Environment: Closed working environment to protect the sample and vitrified grid during the vitrification and storage process.

   

Applications: Sample preparation instrument for rapidly vitrifying several samples for cryoEM data analysis. 

Specifications:

• Chamber Size - 106 x 90mm
• Plasma Current - 0 to 30 mA
• Vacuum Range - 1.1 to 0.2 mbar

Applications: TEM grid surfaces are typically hydrophobic. Glow discharge treatment in air introduces negative charges on the carbon film surface, making it hydrophilic and allowing aqueous solutions to spread more evenly.

Specifications:

• User Interface: Intuitive touchscreen interface with fully programmable protocols.
• Vacuum System and Stage: Equipped with an oil-free vacuum pump system and a motorized 3-axis stage (standard rotation, with optional tilt and height adjustment), allowing uniform coating over areas larger than 100 mm.
• Thickness Monitoring: Integrated quartz crystal microbalance (QCM) for accurate, real-time film thickness measurement.
• Coating Techniques Supported: Compatible with multiple deposition methods, including sputtering, carbon thread, carbon rod, and e-beam evaporation.

Applications: Carbon coating and glow discharge for cryo-EM grids; thin carbon or metal coatings for TEM and FE-SEM.